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Which technique allows precise insertion of large DNA fragments into genomes?
- PCR
- CRISPR-Cas9
- Recombineering
- Gel electrophoresis
Answer: Recombineering
Recombineering uses phage proteins for homologous recombination in vivo, enabling large DNA insertions/deletions. Complements CRISPR for complex genome engineering in synthetic biology. [[DBT Reports, NCERT Biology Class 12]]